Biochemical Analysis Software for Agilent ChemStation 19
Kinetics Mode 2
• The Use Wavelength field allows you to define the wavelength (for multicell
measurements) or up to six wavelengths (for single cell measurements) of
your processed data from which the amplitude values are extracted for the
wavelength result. The default wavelength is 480 nm. If you enter
wavelengths at which no measured data are available (for example
non-integer values), the amplitude values are calculated by linear
interpolation.
• The Background Correction field allows you to specify any background
correction that is applied to the wavelength result to calculate a function
result. Choose the down arrow and select a background correction
procedure from the drop-down list:
none — specifies that the wavelength result is used as measured without
background correction.
single reference wavelength — specifies that the absorbance at a single
wavelength is subtracted from the wavelength result. You specify the
background wavelength in the field.
subtract average over a range — specifies that the average absorbance over
a wavelength range is subtracted from the wavelength result. You specify
the upper and lower limits of the background wavelength range in the
fields.
three-point drop-line — specifies that a background absorbance calculated
using a three-point drop-line is subtracted from the wavelength result. You
specify the upper and lower limits of the background wavelength range
used for the background calculation in the adjacent left (lower) and right
(upper) fields.
• The Trace Monitor is a continuously-updated time trace that shows the
change of absorbance at the wavelength specified in the Use Wavelength
field with time. Define the scaling of the absorbance (y) axis by typing lower
and upper limits in the Y scaling from: and to: fields.
• You can choose to monitor either the last acquired spectrum or spectra
from a specific cell. Choose the down arrow and select either Last
Spectrum, Cell n (for multicell measurements) or All Spectra (for single cell
measurements). Define the scaling of the absorbance (y) axis by typing
lower and upper limits in the Y scaling from: and to: fields.
• The Run Time is the total time of the analysis. The Run Time range is up to
999999 seconds (11.5 days). The Run Time must be greater than Cycle Time
plus Start Time.
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