20 Biochemical Analysis Software for Agilent ChemStation
2 Kinetics Mode
• The Start Time is the time between initiating measurement and
measurement starting, and is optional. The default value is 0.0.
• The Cycle Time is the time from the start of one measurement to the start of
the next; the default value is 5.0 seconds for multicell operation, 0.5
seconds for single cell operation (minimum acceptable values).
• The minimum cycle time is hardware-dependent, and the value given is an
approximation only. To avoid losing data, you must determine the minimum
cycle time for your configuration experimentally. The minimum cycle time
depends on the sampling system (number of measurements per cycle), the
integration time (defined in the Setup Spectrophotometer… dialog box) and
the stray light correction (defined in the Info & Options dialog box).
• Select Increment cycle time to activate the increase in cycle time. Specify
the percentage increase in the % field and a time interval (in seconds) in the
after initial time of: field.
• You can choose from five different kinetic evaluation types. Choose the
down arrow and select the calculation type from the drop-down list:
None — specifies that no rate calculation is made.
Initial rate — specifies that a quadratic fit is made according to the
equation, a+bt+ct
2
where b is the initial rate constant.
Zero order — specifies that a linear fit is made according to the equation,
a + bt where b is the rate constant in AU/s.
First order — specifies that an exponential fit is made according to the
equation,
a+b·exp(-kt) where k is the rate constant in 1/s.
Delta AU — specifies a subtraction of the starting absorbance from the final
absorbance:
Abs
t(end)
- Abs
t(start)
• You can specify that the rate calculation is made over a limited time range.
Type the start time for the calculation in the from: field and the end time in
the to: field.
• Select Multiply Rate to activate a conversion factor for the rate result.
Specify the factor and the new units in the to convert to Rate unit: field.
Select Subtract Rate of Cell to subtract the result of a reference cell from the
rate results of all other cells. Choose the down arrow and select the reference
cell from the drop-down list. This option is available only when you have
configured a multiple-cell measurement.
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