HT2800T_en_c.doc Page 139 of 275
•
Shaker On: interval time during incubation time in which the sample is shaken;
•
Shaker Off: interval time during incubation time in which the sample is not shaken.
Select “Exit” to exit from this screen and return to the “RUN: METHOD X” screen (see
paragraph 5.1.2 “Method X: Setting method parameters”“).
5.1.2.4 Sample parameters
Access sequence: RUN>SETTINGS>METHODS>METHOD X>SAMPLE
The following parameters can be viewed/edited:
•
Sample Volume: volume of sample to be injected;
•
Fill Volume: the volume that the syringe dispenses inside the vial if the “Syringe Prefill“
has been selected as “YES”, or the volume that the syringe aspirates and dispenses
from the vial during the Pull Up Strokes (if the Pull Up Strokes number set is different
from zero);
•
Pull Up Strokes: the number of pre-aspirate strokes (during each stroke the “Fill
Volume” is aspirated and ejected). This procedure assures a good mix of the sample.
•
Equilibration Delay: delay time between sample pick up and the syringe moving from
the sample;
•
Sample speed: speed of the plunger during the syringe filling operation;
•
Syringe Prefill: syringe-filling mode. You can choose if, when the needle comes in the
vial, the syringe plunger has to be pulled up (YES) or pushed down (NO), before the
septum has been pierced.
Select “Exit” to exit from this screen and return to the “RUN: METHOD X” screen (see
paragraph 5.1.2 “Method X: Setting method parameters”“).
5.1.2.5 Injection parameters
Access sequence: RUN>SETTINGS >METHODS>METHOD X>INJECTION
The following parameters can be viewed/edited:
•
Injection speed: the syringe dispensation speed used during injection;
•
Pre Injection Dwell: the waiting time of the syringe needle inside the injection port
before making the injection;
•
Post Injection Dwell: is the waiting time of the syringe needle inside the injector after
the injection;
•
Flush Time: syringe flush time after the injection.
Select “Exit” to exit from this screen and return to the “RUN: METHOD X” screen (see
paragraph 5.1.2 “Method X: Setting method parameters”“).
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