HT2800T_en_c.doc Page 79 of 275
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Prepare the sample vial according to the instructions given in the paragraphs below;
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Put the vial in the rack.
3.7.1 Vial filling in liquid mode
This paragraph contains suggestions about the vial filling in liquid mode.
The recommended fill volume is up to a maximum 50% of the vial nominal volume (e.g.: 1ml for
2ml vial; 50ul for 100ul vial). The air space in the vial is necessary to avoid vacuum formation
during sample aspiration as this could affect reproducibility.
1ml
50l
2ml vial 100ul vial
Figure 93: Recommended fill volumes for sample vials
When preparing sample vials remember that:
•
If you want to test a large sample over repeated injections, divide the sample in different
vials to obtain more reliable data;
•
If the sample volume in the vial is low, contaminants from the previous sample or solvent
washes could have a bigger impact on the sample.
If you change vial supplier, you may need to recheck your method. Differing manufacturing practices
for vial cause sometimes variances in analytical data.
3.7.2 Vial capping (Screw Cap)
If using a sample vial with a screw cap top, firmly tighten the cap ensuring that the septum is flat and
not twisted.
3.7.3 Vial capping (Crimping Cap)
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