C-2 Calibration Reference Information
Overview
Calibration reference files and DRE file consist of two columns of numbers
separated by any number of spaces or characters. The first column contains
the reference peak masses (m/z), and the second column contains the
reference peak intensities.
Most samples can be purchased from the Sigma chemical company. To order
them, contact Sigma at http://www.sigma.sial.com. This site includes a list of
worldwide Sigma offices, many with local toll-free numbers.
FC-70 can be purchased from Fluorochem at http://www.fluorochem.net.
Editing a calibration reference file
Calibration reference files (*.ref) are stored in the MassLynx/Ref folder.
Calibration reference files are created or edited using any Windows® text
editor like Notepad.
To save the reference file after editing, click File > Save to save the file under
the current name, or click File > Save As to save as a new reference file with a
new name.
Textual information or comments can be stored in the reference file. Lines
that are textual information or comments must start with the semicolon (;)
character.
Calibration reference file
Heptacosa
Use the heptacos.ref file for calibration in EI+.
Heptacos.ref file:
Calculated
m/z value
Intensity
Calculated
m/z value
Intensity
Calculated
m/z value
Intensity
49.99680 0.73 130.99199 36.31 213.99030 0.76
68.99518 100.00 149.99039 1.29 218.98560 38.07
92.99518 0.56 163.99350 0.56 225.99030 0.56
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