Zeiss Axio Scope.A1 - F Ig. 4-5 Centering the Annular Phase Diaphragm (Light-Colored, in the Condenser) and the Phase Ring (Dark-Colored, in the Object); Fig

OPERATION
Axio Scope.A1 Illumination and Contrasting Method Carl Zeiss
M60-2-0007 e 05/08 75
• If the overlap is not exact (
632H632HFig. 4-5/A), the
lighter annular diaphragm must be recentered
with the aid of two hexagon screwdrivers
SW 1.5 (
633H633HFig. 4-4/1 and 4). Adjust the two
centering screws (
634H634HFig. 4-4/2 and 3) so that you
achieve a full overlap with the darker phase ring
(
635H635HFig. 4-5/B).
• Remove the auxiliary microscope from the tube
and replace it with the eyepiece.
In order to increase the image contrast, an
interference broadband filter, green 32 x 4, may be
mounted on the field diaphragm or inserted into
the color glass carrier (if available).
A complete phase contrast can only be achieved
when the light-colored annular diaphragm (in the
condenser) overlaps exactly with the dark-colored
phase ring (in the object) in the illumination beam
path (
636H636HFig. 4-5/B).
Fig. 4-5 Centering the annular phase
diaphragm (light-colored, in the
condenser) and the phase ring
(dark-colored, in the object)