Zeiss Axio Scope.A1 - Page 84

OPERATION
Carl Zeiss Illumination and Contrasting Method Axio Scope.A1
84 M60-2-0007 e 05/08
• Adjust the knurled knob on the field diaphragm (
663H663HFig. 4-11/4) so that the field diaphragm becomes
visible in the field of view (
664H664HFig. 4-11/A).
• Readjust the focus on the edge of the field diaphragm with the focusing drive (
665H665HFig. 4-11/B) and center
the field diaphragm on the edge of the field of view (
666H666HFig. 4-11/C) with the centering screws (667H667HFig.
4-11/3).
• Now open the field diaphragm enough to just make it disappear behind the edge of the field of view
(
668H668HFig. 4-11/D).
• For adjusting the aperture diaphragm (image contrast) take out an eyepiece from the tube barrel and
look into the barrel or mount an auxiliary microscope instead of the eyepiece.
• Center the aperture diaphragm with the centering screws (
669H669HFig. 4-11/1) and adjust the knurled knob
(
670H670HFig. 4-11/2) to about 2/3 to 4/5 of the diameter of the objective exit pupil (671H671HFig. 4-11/E) for samples
with a medium contrast quality.
In most cases this position of the aperture diaphragm gives the best contrast at almost full resolution
which is best compromise for the human eye.
• Replace the eyepiece and adjust the focus of the coaxial rough and fine drive and the image
brightness according to the reflected light sample.
Never use the aperture diaphragm to regulate the image brightness! Use the control knob on
the auxiliary power supply unit to adjust the light intensity!