OPERATION
Axio Scope.A1 Illumination and Contrasting Method Carl Zeiss
M60-2-0007 e 05/08 89
The values a and b (see
684H684HFig. 4-14) are determined
with the aid of the eyepiece graticule micrometer
or with the micrometer eyepiece.
If you work in the white light (without interference
filter), you must set λ = 550 nm. When
interference filters are used, it is important to apply
the focal point of their wave lengths.
The measured path difference depends on the
aperture and increases with the illumination
aperture.
The following correction values must be considered depending on the used objective:
Objective Correction factor k
5x/0.15 1.0057
10x/0.25 1.0161
10x/0.30 1.0236
20x/0.4 1.0436
20x/0.50 and 50x/0.75 1.0718
50x/0.60 1.1111
50x/0.75 and100x/0.75 1.2038
50x/0.80 1.2500
50X/0.90 and100x/0.90 1.3929
100X/0.95 1.5241
Table 1: Correction depending on aperture
Example:
a = 11 mm b = 5 mm
λ = 550 nm objective 20x/0.50
nm134
mm22
0718.1mm5nm550
a2
kb
d =
⋅⋅
=
⋅⋅λ
=
b
a
Fig. 4-14 Interference stripes
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