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BD FACSAria
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114 BD FACSAria User’s Guide
5 Reconnect the sensor and quick-release connector to their respective ports,
if needed.
Connect the lines if they were disconnected in step 1. See Figure 4-2 on
page 113.
6 Prime the fluidics system if you disconnected the fluid lines in step 1.
From the BD FACSDiva menu bar, select Cytometer > Cleaning Modes >
Prime after Tank Refill. In the dialog box, select the fluid to prime, then
click OK.
Emptying the Waste
Empty the waste tank daily and when the fluid indicator shows the waste is
getting full. To prolong the life of the tank, BD Biosciences recommends that you
switch to the alternate tank each time the waste is emptied.
To ensure that the appropriate solutions are dispensed, do not switch the
tank positions. Make sure the label on each container matches the labelled
port on the fluidics cart.
All biological specimens and materials coming into contact with them can
transmit potentially fatal disease. To prevent exposure to biohazardous
agents, expose waste container contents to bleach (10% of total volume)
before disposal. Dispose of waste in accordance with local regulations. Use
proper precautions and wear suitable protective clothing, eyewear, and
gloves.
Change the waste tank cap every month to prevent tank pressurization. To
order new replacement caps (BD Part No. 33885407, pack of 12), contact
your local BD Biosciences representative.

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