Chapter 1: BD FACSAria Cytometer Components 39
Detectors
A standard system is equipped with an octagon containing six PMTs, which
detect light from the 488-nm (blue) laser, and a trigon containing two PMTs,
which detect light from the 633-nm (red) laser. The third-laser option adds an
additional trigon containing two PMTs to detect light from the 405-nm (violet)
laser. Optical upgrades are available to fill the remaining PMT slots for a two- or
three-laser system. A fully-equipped system can detect up to 13 fluorescent
parameters and two scatter parameters at a time.
Figure 1-16 Fully loaded detector arrays
At installation, the octagon and trigon arrays are set up with the filter and mirror
combinations shown in Table 1-1 on page 40. You can rearrange these
configurations according to the type of fluorochromes in your experiment. See
Application Options on page 250 for more information.
octagon
trigons
blue-laser
red-laser
signal
violet-laser
signal
signal
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