Roche Diagnostics
Operator’s Manual · Version 3.0 159
Multiplate® analyzer 6 Configuration
Editing test parameters
Definitions for tests using the
electronic pipette
Up to three pipette steps may be incorporated into a test program both before and
after incubation by entering the volume in μL and the name of the liquid. Steps with
no value or with the value 0 (zero) are skipped during execution.
q
o The minimal volume for steps 1–6 is 10 μL. A minimum of 600 μL must be in the test
cell to start a measurement. The total volume for one test cell must be between 350
and 1000 μL.
o If the volume is set to 0 μL for steps 4–6, the test cannot be executed.
Incubation time The default value is 180 seconds.
Measuring time during incubation The time in seconds when the measuring will start before the end of the incubation.
Enter a time that is shorter or equal to the incubation time.
Example:
you enter 30 and the incubation time is set to 180 seconds. The measuring will start
after 150 seconds counted from the incubation start.
Test duration Duration of the test in minutes. Default test duration is 6 minutes. Enter a value
between 3 and 30 minutes.
Pipetting aspiration speed
Pipetting dispensing speed
Roche recommends not to change the default pipetting speed.
Default value is 5. The value may be changed for research purposes between 1 and 9.
Reference ranges Reference ranges can be applied for each parameter:
o Aggregation AU
o Velocity AU/min
o Area under the curve (AUC) AU*min / U
q
When programming a new test, reference values must be entered in AU*min. If the AUC is
to be displayed in U, the software automatically converts the AU*min values into Units (U).
Because 1U = 10 AU*min, reference range values that have been determined in U must be
entered by adding a zero (multiplied by 10) to the values to convert them into AU*min
values.
Targe t rang e A target range can be applied for the parameter AUC.
q
Target range values must be entered in AU*min. If the AUC is to be displayed in U, the
software automatically converts the AU*min values into Units (U). Because 1U = 10
AU*min, target range values that have been determined in U must be entered by adding a
zero (multiplied by 10) to the values to convert them into AU*min values.
Display definitions To display reference ranges on the results screen and the printouts the values must be
specified in the corresponding boxes.
Select Show reference range for Ag to show the entered reference range for
aggregation in brackets after the current aggregation value AU (B) and as horizontal
green lines within the graphic window (A).
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