Introduction to the Fluorescence Detector 7
1100 Series FD Reference Manual 253
Processing of Raw Data
If the lamp flashes at single wavelength and high-power, then the fluorescence
data rate is 296 Hz. That means that your sample is illuminated 296 times per
second, and any luminescence generated by the components eluted from the
column is measured 296 times per second.
If the “economy” or multi-wavelength mode is set, then the flash frequency is
74 Hz.
You can improve the signal-to-noise characteristics by disabling the “economy”
mode.
The data resolution is 20 bit at a response time of 4 seconds (default, which is
equivalent to a time constant of 1.8 seconds and appropriate for standard
chromatographical conditions). Weak signals may cause errors in
Figure 85 LAMP: Frequency of Flash, Fluorescence, and Phosphorescence
NOTE
Disabling the “economy” mode will shorten the lifetime of the lamp significantly. Consider
lifetime saving by switching off the lamp after the run is completed.
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