1100 Series FD Reference Manual 5
Contents
1 Installing the Fluorescence Detector
Site Requirements 14
Physical Specifications 16
Unpacking the Detector 17
Detector Accessory Kit Contents 18
Optimizing the Stack Configuration 20
Installing the Detector 22
Flow Connections to the Detector 24
2 First Steps with the Fluorescence Detector
Before You Start 30
Optimization Overview 31
Getting Started and Checkout 33
Starting Your Detector 33
Setting the Chromatographic Conditions 34
Observe the maxima via the isoabsorbance plot 36
Method Development 37
Step 1: Check the LC system for impurities 38
Step 2: Optimize limits of detection and selectivity 39
Step 3: Set up routine methods 48
Example: Optimization for Multiple Compounds 52
3 Optimizing the Fluorescence Detector
Optimization Overview 62