250 1100 Series FD Reference Manual
7 Introduction to the Fluorescence Detector
Reference System
A reference diode, located behind the flow cell, measures the excitation (EX)
light transmitted by the flow cell and corrects flash lamp fluctuations and
long-term intensity drift. Because of a non-linear output of the diode
(depending on the EX-wavelength), the measured data are normalized.
A diffuser is located in front of the reference diode (see Figure 77 on
page 244). This diffuser is made of quartz, reduces light and allows integral
measurement of the light.