Agilent Technologies 1100 Series Reference Manual

Optimizing the Fluorescence Detector 3
1100 Series FD Reference Manual 65
A Real Example
Although an excitation wavelength of 340 nm is quoted in the literature the
Agilent 1100 fluorescence detector scan of orthophthalaldehyde, a derivative
of the amino acid alanine, (Figure 26 on page 65) shows a maximum between
220 nm and 240 nm.
When you are looking for the wavelength by scanning, scan over the whole
range. As this example shows a maximum may be found in a completely
different wavelength range.
Figure 26 Scan Orthophthalaldehyde Derivative of Alanine
NOTE
When comparing fluorescence excitation spectra directly with DAD spectra or literature
based absorbance spectra, you should consider large differences in the used optical
bandwidth (FLD=20 nm) which cause a systematic wavelength maximum shift depending
on the absorbance spectrum of the compound under evaluation.

Agilent Technologies 1100 Series Specifications

General

Brand	Agilent Technologies
Model	1100 Series
Category	Laboratory Equipment
Language	English