Introduction to the Fluorescence Detector 7
1100 Series FD Reference Manual 251
Analytical Information From Primary Data
We now know how the primary data from your sample is acquired in the
optical unit. But how can the data be used as information in analytical
chemistry? Depending on the chemistry of your application, the luminescence
measured by the fluorescence detector will have different characteristics. You
must decide, using your knowledge of the sample, what mode of detection you
will use.
Fluorescence Detection
When the lamp flashes, the fluorescing compounds in your sample will
luminesce almost simultaneously, see Figure 83. The luminescence is
short-lived, therefore the fluorescence detector need only measure over a
short period of time after the lamp has flashed.
Figure 83 Measurement of Fluorescence
12 30
Time [µsec]
Intensity
100%
Ignite
Track&Hold