52 1100 Series FD Reference Manual
2 First Steps with the Fluorescence Detector
Example: Optimization for Multiple Compounds
Using PNAs as a sample, this example uses the described scanning functions.
Setting the Chromatographic Conditions
1 This example uses the following chromatographic conditions (the detector
settings are shown in Figure 18 on page 53).
Tabl e 12 Chromatographic Conditions
Mobile phases A = water = 50%
B = Acetonitrile = 50%
Column Vydac-C18-PNA, 250 mm x 2.1 mm i.d. with 5 µm
particles
Sample PAH 0.5 ng
Flow rate 0.4 ml/min
Compressibility A (water) 46
Compressibility B (Acetonitrile) 115
Stroke A and B auto
Time Table at 0 minutes %B=50
at 3 minutes %B=60
at 14.5 minutes %B=90
at 22.5 minutes %B=95
Stop time 26 minutes
Post time 8 minutes
Injection volume 1 µl
Oven temperature (1100) 30°C
FLD PMT Gain PMT = 15
FLD Response time 4 seconds
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