First Steps with the Fluorescence Detector 2
1100 Series FD Reference Manual 51
In addition, the purity of a peak can be investigated by comparing spectra
obtained within a single peak. When a peak is calculated to be within the
user-defined purity limits, the purity factor is the mean purity value of all
spectra that are within the purity limits.
The reliability of the purity and the match factor depends on the quality of
spectra recorded. Because of the lower number of data points available with
the fluorescence detector in general, the match factors and purity data
obtained show stronger deviations compared to data from the diode array
detector, even if the compounds are identical.
Table 11 shows an automated library search based on the emission spectra
from a PNA reference sample.
Tabl e 11 Peak confirmation using a fluorescence spectral library
Meas. Library CalTbl
RetTime
Signal Amount Purity # Match Libary Name
[min] [min] [min] [ng] Factor
4.859 4.800 5.178 1 1.47986e-1 - 1 993 Naphthalene@em
6.764 7.000 7.162 1 2.16156e-1 - 1 998 Acenaphthene@em
7.137 7.100 7.544 1 1.14864e-1 - 1 995 Fluorene@em
8.005 8.000 8.453 1 2.56635e-1 - 1 969 Phenanthrene@em
8.841 8.800 9.328 1 1.76064e-1 - 1 993 Anthracene@em
9.838 10.000 10.353 1 2.15360e-1 - 1 997 Fluoranthene@em
10.439 10.400 10.988 1 8.00754e-2 - 1 1000 Pyrene@em
12.826 12.800 13.469 1 1.40764e-1 - 1 998 Benz(a)anthracene@em
13.340 13.300 14.022 1 1.14082e-1 - 1 999 Chrysene@em
15.274 15.200 16.052 1 6.90434e-1 - 1 999 Benzo(b)fluoranthene@em
16.187 16.200 17.052 1 5.61791e-1 - 1 998 Benzo(k)fluoranthene@em
16.865 16.900 17.804 1 5.58070e-1 - 1 999 Benz(a)pyrene@em
18.586 18.600 19.645 1 5.17430e-1 - 1 999 Dibenz(a,h)anthracene@em
19.200 19.100 20.329 1 6.03334e-1 - 1 995 Benzo(g,h,i)perylene@em
20.106 20.000 21.291 1 9.13648e-2 - 1 991 Indeno(1,2,3-cd)pyrene@em
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