Optimizing the Fluorescence Detector 3
1100 Series FD Reference Manual 63
Design Features Help Optimization
The Agilent 1100 fluorescence detector has several features you can use to
optimize detection:
Check Performance Before You Start
Before you start you should check that your detector is performing according
to the specifications published by Agilent Technologies.
Your normal LC grade solvents may give good results most of the time but our
experience shows that baseline noise can be higher with LC grade solvents
than with fluorescence grade solvents.
Flush your solvent delivery system for at least 15 minutes before checking
sensitivity. If your pump has multiple channels, you should also flush the
channels not in use.
Tabl e 15
PMTGAIN Amplification factor
LAMP Flash frequency
RESPONSETIME Data reduction interval
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