Agilent Technologies 1100 Series - Observe the Maxima Via the Isoabsorbance Plot

36 1100 Series FD Reference Manual
2 First Steps with the Fluorescence Detector
Observe the maxima via the isoabsorbance plot
1 Load the data file (λ
EX
=246 nm, λ
EM
=317 nm) and open the isoabsorbance
plot.
2 The maximum
λ
EX
will be found around 250 nm.
Figure 9 Isoabsorbance Plot