Troubleshooting and Test Functions 4
1100 Series FD Reference Manual 101
Wavelength Verification and Calibration
The wavelength calibration is based on a Glycogen solution, which acts as a
strong elastic light scatterer (refer to ASTM Test Method E388-72-1993
“Spectral Bandwidth and Wavelength Accuracy of Fluorescence
Spectrometers”). The Glycogen solution is introduced into the flow cell and
then the built-in wavelength calibration functionality is used.
The algorithm is based on evaluating different grating orders and calculating
the wavelength scales of both, excitation and emission monochromator, by
applying the fundamental grating equation.
( X ) only required, if deviation is too large.
NOTE
A complete wavelength calibration is not always required. In most cases a quick
wavelength accuracy verification is sufficient enough, see the table below.
Tabl e 18 Reasons for doing a Verification or Calibration
Verification WL calibration
interest X
GLP compliance X
cell change X ( X )
lamp change X ( X )
monochromator change X
main board change X
optical unit change X
NOTE
Prior to a wavelength calibration, a wavelength accuracy verification should be performed,
see “Check of Raman Band of Water" on page 104. If the deviation is more than
±3nm, the
wavelength calibration should be done as described in “The Wavelength Calibration
Procedure" on page 110.
To Next Page
Loading...
Need help?
General
