102 1100 Series FD Reference Manual
4 Troubleshooting and Test Functions
Table 19 on page 103 shows the steps performed during the wavelength
calibration.
The excitation grating and the emission grating are calibrated using Rayleigh
stray light from the flow cell or cuvette measured with the photomultiplier
tube.
NOTE
The duration of the wavelength calibration is about 15 minutes plus setup time for the
calibration sample and system. Depending on the maximum intensity found during this
scan, the PMT gain will be changed automatically and requires an additional 1 minute per
scan.
Figure 33 Wavelength Calibration - User Interface
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