Agilent Technologies 1100 Series - Page 45

First Steps with the Fluorescence Detector 2
1100 Series FD Reference Manual 47
Figure 15 UV-spectrum and fluorescence spectra for 2,3-diaminophenazine (DAP)
Figure 16 Qualitive analysis of MBC (2-benzimidazole carbamic acid methylester) and
impurities
This is an impurity of
carbamates. The
excitation spectrum in
a second run shows
the equivalence of
UV-spectra and
fluorescence
excitation spectra. An
excitation wavelength
at 265 nm was used
for taking the
emission spectrum
and an emission
wavelength at 540 nm
was used for taking
the excitation
spectrum.
The two upper traces
are obtained using
two different
excitation
wavelengths. The
lower trace is a pure
standard of the
known impurities.