Chapter 4: Running Samples 121
Preparing QC Particles
Prepare QC particles, also known as beads, in a 12 x 75-mm tube according to
the manufacturer’s instructions. See Table 4-2 for one brand of beads that can be
ordered from BD.
Table 4-2 Bead Information
Adjusting Area Scaling and Laser Delay
The required area scaling factor changes based on sheath pressure and particle
size. The area scaling factors should be verified for each experiment performed
on the cytometer.
This section describes how to optimize area scaling and laser delay using the QC
template that is included with BD FACSDiva software.
Setting Up the QC Experiment
Follow the steps in this section to set up an experiment for instrument QC.
1 Click the corresponding buttons on the Workspace toolbar to display the
Browser ( ), Cytometer ( ), Inspector ( ), Worksheet ( ), and
Acquisition Dashboard ( ) windows, as needed.
Tip As you work in the software, windows can become hidden. You can
easily bring a window to the forefront by double-clicking the corresponding
button on the Workspace toolbar.
BD FACSFlow™
Solution
SPHERO Rainbow Particles
(3.0–3.4 μm)
Ordering Info
1 mL 2 to 3 drops BD Biosciences Cat. No. 556291
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