216 BD FACSAria User’s Guide
7 Remove the sheath line from the filter purge tubing and reconnect the
sheath line to the filter outlet tubing as shown in Figure 6-10.
8 Turn on the stream and ensure that there are no bubbles in the flow cell.
9 Repeat the stream-off and stream-on modes as necessary until all bubbles
are removed from the flow cell.
10 Increase the sheath pressure to its original value.
Select Cytometer > Sheath Pressure and input the setting from step 1.
NOTE If the cytometer is run until the sheath supply is depleted, air will
accumulate in the bubble filter. Purge the filter as described in Purging the Bubble
Filter on page 214. If you are unable to remove all of the air, replace the bubble
filter as described in Changing the Bubble Filter on page 203.
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