258 BD FACSAria User’s Guide
Application Settings
Application settings are associated with a cytometer configuration and include
the parameters needed for the application, area scaling values, PMT voltages, and
threshold values, but not compensation. Each time a performance check is run
for a configuration, the application settings associated with that configuration
are updated to the latest run.
Using application settings provides an easy, consistent, and reproducible way to
reuse cytometer settings for your commonly used applications.
Before creating application settings you must:
• Perform the cytometer startup procedure described in Cytometer Startup
on page 108.
• Run a performance check for the cytometer configuration that will be used
for the application.
Creating Application Settings
This section describes how to create and save application settings for a multi-
color immunophenotyping sample.
Setting Up the Workspace
1 Create a new folder.
2 Create a new experiment.
3 Select the Cytometer Settings in the Browser.
4 In the Inspector window, select the Parameters tab, and delete unwanted
parameters.
5 Click in the H checkbox to select Height for each parameter. See
Figure B-1.
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