66 BD FACSAria User’s Guide
Typically, when setting up for sorting, you use the Amplitude to set the required
drop breakoff, and copy the generated Drop 1 value to the target field. Then, you
turn on the Sweet Spot to maintain the drop breakoff. When the Sweet Spot
detects a >1-pixel difference between the target Gap setting and the actual Gap, it
adjusts the Amplitude to reduce the Gap. When a >2-pixel difference is detected,
the Sweet Spot stops sorting temporarily (stops charging the stream) until the
Gap is restored. Once the Gap is back within range, sorting automatically
resumes.
For information on setting the breakoff, see Setting Up the Breakoff on page 110.
Frequency field
(continued)
In general, the drop drive frequency should not need adjustment.
BD Biosciences recommends using the default values that are entered
with each Sort Setup mode.
Drop 1 field The distance between the top of the image and the center of the first
broken-off drop, from 100–600 pixels.
When you input a value and turn on the Sweet Spot, the cytometer
automatically adjusts the amplitude to attain your target value.
Note that not all Drop 1 targets are attainable. The Drop 1 value
jumps in whole increments of approximately 57 pixels according to
your drop spacing.
The same Drop 1 setting should be used from day to day. A difference
of up to 10 pixels between the target value and the actual value is
acceptable.
Gap field The gap between the stream breakoff and the top of the first drop.
The default Gap setting is 6 pixels, and in general, it should never be
adjusted. Differences of 1–2 pixels between the target value and the
cytometer-generated value are acceptable.
Table 2-1 Breakoff window controls (continued)
Control Description
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