First Steps with the Fluorescence Detector 2
1100 Series FD Reference Manual 41
that are going to be analyzed in the chromatographic run and the background
noise that may be different upon excitation at 250 nm, 315 nm or 350 nm. The
maximum of emission is observed at 440 nm.
Details for Figure 12 on page 42:
All excitation and emission spectra of Quinidine (1 µg/ml) are shown in
graphic. Fluorescence intensity is plotted vs excitation and emission
wavelengths.
Detector settings: step size 5 nm, PMT 12, Response time 4 s