17 Chromatographic Checkout
244 Operation Manual
To Check TCD Performance
•
1 Gather the following:
• Evaluation column, HP-5 30 m × 0.32 mm × 0.25 µm (19091J-413)
• FID/TCD performance evaluation (checkout) sample (18710-60170)
• 4-mL solvent and waste bottles or equivalent for autoinjector
• Chromatographic-grade hexane
• 2-mL sample vials or equivalent for sample
• Chromatographic-grade helium as carrier, makeup, and reference gas
• Inlet and injector hardware See “To Prepare for Chromatographic Checkout.”
2 Verify the following:
• Chromatographic-grade gases plumbed and configured: helium as carrier gas and
reference gas.
• Empty waste vials loaded in sample turret.
• 4-mL solvent vial with diffusion cap filled with hexane and inserted in Solvent A injector
position.
3 Replace consumable parts (liner, septum, traps, syringe, and so forth) as needed for the
checkout. See “To Prepare for Chromatographic Checkout.”
4 Install the evaluation column.
• Bake out the evaluation column for at least 30 min at 180 °C.
• Configure the column
5 Create or load a method with the parameter values listed in Table 43.
Table 43 TCD Checkout Conditions
Column and sample
Type HP-5, 30 m × 0.32 mm × 0.25 µm
(19091J-413)
Sample FID/TCD checkout 18710-60170
Column flow 6.5 mL/min
Column mode Constant flow.
Split/splitless inlet
Temperature 250 °C
Mode Splitless
Purge flow 60 mL/min
Purge time 0.75 min
Septum purge 3 mL/min
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