5Methodsï€
Operation Manual 85
Isothermal runs
An isothermal run is one in which the oven is maintained at a constant temperature. For an
isothermal run, set Rate 1 to zero.
1 Select Oven to open the oven parameter list.
2 Enter the oven temperature for the isothermal run.
3 Enter the number of minutes (Initial time) that you want the oven to stay at this
temperature. This time is the duration of the run.
4 If Rate 1 is not already 0, enter zero for an isothermal run
Oven cryogenic cooling parameters
All cryogenic setpoints are found in Method > Oven.
Cryo Cooling Select or deselect this checkbox to enable or disable cryogenic cooling
respectively.
Quick cool This feature is separate from Cryo. Quick cool makes the oven cool faster after a
run than it would without assistance. This feature is useful when maximum sample
throughput is necessary, however it does use more coolant. Quick cool turns off soon after the
oven reaches its setpoint and Cryo takes over, if needed.
Ambient Temperature The temperature in the laboratory. This setpoint determines the
temperature at which cryogenic cooling is enabled:
• Ambient temp + 25°C, for regular cryo operation
• Ambient temp + 45°C, for Quick Cryo Cool.
Timeout Detection Cryo timeout occurs, and the oven shuts off, when a run does not start
within a specified time (10 to 120 minutes) after the oven equilibrates. Turning cryo timeout off
disables this feature. We recommend that it be turned on because cryo timeout conserves
coolant at the end of a sequence or if automation fails. A Post Sequence method could also be
used.
70 to 115 45 95 95 120
115 to 175 40 65 65 110
175 to 300 30 45 45 80
300 to 450 20 35 35 65
Table 4 Oven ramp rates (continued)
100/120 V oven ï€
ramp rate (°C/minute)
200/220/230/240 V oven ï€
ramp rate (°C/minute)
Temperature
range (°C)
Without insert With optional
insert
Without insert With optional
insert
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