17 Chromatographic Checkout
Operation Manual 239
To Check FID Performance
1 Gather the following:
• Evaluation column, HP-5 30 m × 0.32 mm × 0.25 µm (19091J-413)
• FID performance evaluation (checkout) sample (5188-5372)
• Chromatographic-grade isooctane
• 4-mL solvent and waste bottles or equivalent for autoinjector
• 2-mL sample vials or equivalent for sample
• Inlet and injector hardware (See “To Prepare for Chromatographic Checkout.”)
2 Verify the following:
• Capillary column jet installed. If not, select and install a capillary column jet.
• Capillary column adapter installed. If not, install it.
• Chromatographic-grade gases plumbed and configured: helium as carrier gas, nitrogen,
hydrogen, and air.
• Empty waste vials loaded in sample turret.
• 4-mL solvent vial with diffusion cap filled with isooctane and inserted in Solvent A
injector position.
3 Replace consumable parts (liner, septum, traps, syringe, and so forth) as needed for the
checkout. See “To Prepare for Chromatographic Checkout.”
4 Install the evaluation column.
• Bake out the evaluation column for at least 30 min at 180 °C.
• Be sure to configure the column.
5 Check the FID baseline output. The output should be between 5 pA and 20 pA and
relatively stable. (If using a gas generator or ultra pure gas, the signal may stabilize below
5 pA.) If the output is outside this range or unstable, resolve this problem before
continuing.
6 If the output is too low:
• Check that the electrometer is on.
• Check that the flame is lit.
• Check that the signal is set to the correct detector.
7 Create or load a method with the parameter values listed in Table 42.
Table 42 FID Checkout Conditions
Column and sample
Type HP-5, 30 m × 0.32 mm × 0.25 µm
(19091J-413)
Sample FID checkout 5188-5372
Column flow 6.5 mL/min
Column mode Constant flow.
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