17 Chromatographic Checkout
248 Operation Manual
To Check NPD Performance
1 Gather the following:
• Evaluation column, HP-5 30 m × 0.32 mm × 0.25 µm (19091J-413)
• NPD performance evaluation (checkout) sample (18789-60060)
• 4-mL solvent and waste bottles or equivalent for autoinjector.
• Chromatographic-grade isooctane
• 2-mL sample vials or equivalent for sample.
• Inlet and injector hardware See “To Prepare for Chromatographic Checkout.”
2 Verify the following:
• Capillary column jet installed. If not, select and install a capillary column jet.
• Chromatographic-grade gases plumbed and configured: helium as carrier gas, nitrogen,
hydrogen, and air.
• Empty waste vials loaded in sample turret.
• 4-mL vial with diffusion cap filled with isooctane and inserted in Solvent A injector
position.
3 Replace consumable parts (liner, septum, traps, syringe, and so forth) as needed for the
checkout. See “To Prepare for Chromatographic Checkout.”
4 If present, remove any protective caps from the inlet manifold vents.
5 Install the evaluation column.
• Bake out the evaluation column for at least 30 min at 180 °C.
• Be sure to configure the column
6 Create or load a method with the parameter values listed in Table 44.
Table 44 NPD Checkout Conditions
Column and sample
Type HP-5, 30 m × 0.32 mm × 0.25 µm
(19091J-413)
Sample NPD checkout 18789-60060
Column mode Constant flow
Column flow 6.5 mL/min (helium)
Split/splitless inlet
Temperature 200 °C
Mode Splitless
Purge flow 60 mL/min
Purge time 0.75 min
Septum purge 3 mL/min
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