Chapter 4: Running Samples 127
Figure 4-8 Blue laser area scaling before adjustment (left) and after (right)
Adjusting Area Scaling and Laser Delay for the Red and Violet
Lasers
The laser delay factor in BD FACSDiva software is used to realign the signals so
they can be measured and displayed on the same time scale. Signals are aligned
with respect to the blue laser, so the blue laser will have a 0 delay value, the red
laser will have a negative delay value, and the violet laser will have a positive
delay value.
1 Adjust the APC voltage to place the APC-H signal at approximately 100 x
10
3
.
The red laser area scaling and laser delay can be adjusted using any
parameter excited by the red laser.
2 Adjust the delay setting for the red laser, if needed.
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