186 BD FACSAria User’s Guide
Long Clean
Use the Long Clean command to decontaminate, with 10% bleach or 70%
ethanol, the internal sheath path from the fluid line (starting at the outlet of the
fluid selection valves in the fluidics cart) to the nozzle outlet. After
decontamination, the lines are rinsed with DI water.
There are three long clean options: Long Clean with Bleach, Long Clean with
Ethanol, and Clean Bulk Injection Chamber. Use the bleach option to remove
adhesive dyes such as PI, acridine orange, or thiazole orange, or when you want
to decontaminate the sample lines. Use the ethanol option to decontaminate the
lines with ethanol. The remaining option is described in Clean Bulk Injection
Chamber on page 191.
This procedure can take 10–40 minutes, depending on how long you run the
stream to remove trace decontaminants.
1 Ensure fluid levels are adequate. Empty the waste container if it is full.
For example, if you are performing a bleach cleaning procedure, make sure
the bleach tank is full. The long clean requires approximately 90 mL of
cleaning fluid and 110 mL of DI water.
2 Open the flow cell access door and turn off the stream.
3 Bypass the bubble filter.
Consult with your BD representative before running any other cleaning
solutions.
All biological specimens and materials coming into contact with them can
transmit potentially fatal disease. Use 10% bleach to decontaminate the
sheath path. Flushing the lines with 10% bleach is the only procedure
recommended by BD Biosciences for decontaminating the cytometer.
To prevent damage to the bubble filter, do not run bleach or ethanol
through the filter. Bypass the bubble filter as follows.
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