BD FACSAria - Page 194

194 BD FACSAria User’s Guide
3 Using a paper towel to cover the quick-disconnect coupling, disconnect the
sheath line from the filter outlet tubing at the top of the bubble filter
(Figure 6-1 on page 187).
4 Using a paper towel to cover the quick-disconnect coupling, disconnect the
sheath line from the filter inlet coupling at the bottom of the filter
(Figure 6-1 on page 187).
5 Connect the two sheath line couplings together (Figure 6-2 on page 188).
6 Set the sheath pressure back to its original setting.
Preparing the Cytometer
1 Ensure fluid levels are adequate. Empty the waste container if it is full.
This procedure requires approximately 250 mL of bleach or ethanol.
2 Remove the sheath filter on the back of the fluidics cart by pressing the tabs
on each quick-disconnect coupling.
3 Install bypass tubing in place of the sheath filter.
Make sure you reconnect the bubble filter when you are finished running
the cleaning mode.
coupling
coupling