Chapter 6: Shutdown and Maintenance 193
Prepare for Aseptic Sort
Use the Prepare for Aseptic Sort command when you want to decontaminate the
entire sheath path with 70% ethanol or 10% bleach, from the fluid line starting
at the sheath inlet port in the fluidics cart to the nozzle outlet. For the most
complete decontamination, autoclave the sheath container and install a new
sheath filter before sorting. Autoclave the sheath container at 125°C and 15 psi
for 30 minutes with a 7.5-minute warmup and shutdown cycle.
NOTE To decontaminate the sheath probe, use 10% bleach. Do not autoclave
the probe.
The plenum reservoirs can also be removed and cleaned with bleach before you
run this cleaning mode, if required. To do so, run a long clean with ethanol or
bleach as described in Long Clean on page 186, and then clean the plenum
reservoirs as described on page 190.
This procedure takes 10–40 minutes, depending on how long you run the stream
to remove trace decontaminants. It consists of the following four tasks:
• Bypassing the Bubble Filter on page 193
• Preparing the Cytometer on page 194
• Cleaning the Lines with Ethanol or Bleach on page 195
• Replacing the Ethanol or Bleach with Sheath Fluid on page 197
Make sure you perform the entire procedure.
Bypassing the Bubble Filter
1 Turn off the stream and open the flow cell access door.
2 Reduce the sheath pressure to 10 psi.
Do not run bleach or ethanol through the bubble filter, because it will
damage the filter. Bypass the bubble filter as follows.
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