BD FACSAria - Page 232

232 BD FACSAria User’s Guide
No fluorescent signal Current cytometer
configuration different from
optical setup
Verify that the cytometer optics
match the current cytometer
configuration.
Wrong filter installed or filter
not completely seated
Make sure the appropriate filter is
installed for each fluorochrome.
See Application Options on
page 250 for suggestions. Make
sure the filters are pushed all the
way in.
Laser delay is set incorrectly Adjust the laser delay settings. See
Cytometer Quality Control Using
BD FACSDiva Software on
page 118.
Low Area signal Area scaling is too low Adjust area scaling for the
corresponding laser. See
Cytometer Quality Control Using
BD FACSDiva Software on
page 118.
Unexpected events in
plot
Incorrect logic in population
hierarchy
Verify the gating strategy.
Incorrect population(s) in
plot
Right-click the plot and choose
Show Populations. Verify that the
appropriate populations are
displayed.
Incorrect drawing order Verify that the required population
is not hidden by another
population. Right-click the plot
and choose Order Populations by
Count.
Acquisition Troubleshooting (continued)
Observation Possible Causes Recommended Solutions