Chapter 7: Troubleshooting 231
No events in plots after
clicking Acquire Data
(continued)
Uncolored events in plot • Format the plot to display all
events.
• Assign a color to the population
displayed in the plot.
• Verify the population drawing
order.
Current cytometer
configuration different from
optical setup
Verify that the cytometer optics
match the current cytometer
configuration. See Application
Options on page 250.
No sample in the tube Add sample to the tube or install a
new sample tube.
Sample is not mixed properly Increase the Sample Agitation rate.
See Sample Agitation on page 83.
Sample line is clogged Perform a sample line backflush.
See Sample Line Backflush on
page 183. If necessary, change the
sample line.
Threshold not set to the
correct parameter (usually
FSC)
Set the threshold to the correct
parameter for your application.
Multiple threshold
parameters not set correctly
Verify that the correct Boolean
logic (And/Or) was used for the
threshold parameters.
Threshold channel too low or
too high
Adjust the Threshold channel. See
Calculating Compensation on
page 138.
Optical filter(s) not
completely seated
Make sure the filters are pushed all
the way in.
FSC area setting is incorrect Ensure that the FSC-H matches
the FSC-A value.
Acquisition Troubleshooting (continued)
Observation Possible Causes Recommended Solutions
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