Chapter 7: Troubleshooting 233
Erratic event rate Sample aggregates Filter the sample.
Bulk injection O-ring is worn Contact your BD Biosciences
service engineer.
Sample is contaminated Re-stain the sample, making sure
the tube is clean.
Sheath container low Fill the sheath container.
Unexpectedly high event
rate
Threshold channel is too low Adjust the threshold channel. See
Calculating Compensation on
page 138.
Sample is too concentrated Dilute the sample.
Event rate is too high Decrease the flow rate in the
acquisition dashboard.
Bubbles in flow cell Turn off the stream, wait a few
seconds, and turn on the stream
again.
Unexpectedly low event
rate
Sample not adequately mixed Increase the sample agitation rate.
See Sample Agitation on page 83.
Threshold channel is too high Adjust the threshold channel. See
Calculating Compensation on
page 138.
Sample is too dilute Concentrate the sample.
Sample line is clogged or
kinked
Backflush the sample line. See
Sample Line Backflush on
page 183. If necessary, change the
sample line.
Look for visible kinks in the line.
If kinks are found, change the
sample line. See Changing the
Sample Lines on page 200.
Acquisition Troubleshooting (continued)
Observation Possible Causes Recommended Solutions
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