234 BD FACSAria User’s Guide
Unexpectedly low event
rate (continued)
Sample line installed
incorrectly
Verify the sample line installation.
See Changing the Sample Lines on
page 200.
Sample aggregates Filter the sample.
Memory is full Compare the processed event rate
in BD FACSDiva software with the
threshold counter on the
cytometer. If the software event
rate is much lower, quit and then
restart the application.
Distorted parameters or
high CVs
Cytometer settings adjusted
incorrectly
Optimize the scatter parameters.
See Calculating Compensation on
page 138.
Flow rate is too high Decrease the flow rate in the
acquisition dashboard.
Window extension is too low Increase the window extension.
Bubbles in flow cell Turn off the stream, wait a few
seconds, and turn on the stream
again.
Nozzle is clogged or dirty Clean the nozzle as described in
Cleaning the Nozzle on page 209.
Flow cell is dirty Clean the flow cell with a
detergent such as Contrad
®. See
Clean Flow Cell on page 184. Let
the detergent sit for 5 minutes
before turning on the stream.
Poor sample preparation Repeat sample preparation.
Area scaling is too low Verify area scaling. See Adjusting
Area Scaling and Laser Delay on
page 121.
Acquisition Troubleshooting (continued)
Observation Possible Causes Recommended Solutions
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