Chapter 7: Troubleshooting 235
Excessive amount of
debris in plots
Threshold channel is too low Increase the threshold channel. See
Calculating Compensation on
page 138.
Dead cells or debris in sample Examine the sample under a
microscope.
Sample is contaminated Re-stain the sample, making sure
the tube is clean.
High electronic abort
rate (>10% of system
event rate)
Window extension is too high Decrease the window extension.
Threshold channel is too low Increase the threshold channel.
Event rate is too high Decrease the flow rate in the
acquisition dashboard.
Sample is aggregated Filter the sample.
Sample is too concentrated Dilute the sample.
Fewer events than
expected in gated
population
Window extension set
incorrectly
Adjust the window extension, if
needed. Refer to the
BD FACSDiva Software Reference
Manual for information.
Laser delay set incorrectly Adjust the laser delay settings. See
Cytometer Quality Control Using
BD FACSDiva Software on
page 118.
Plot is zoomed Unzoom the plot or make the gate
bigger.
Events left out of the gate When drawing a gate, make sure
events on the axis are included.
Acquisition Troubleshooting (continued)
Observation Possible Causes Recommended Solutions
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