Appendix A: Supplies and Options 253
Changing Filters
When you use PerCP rather than PerCP-Cy5.5 in the Blue B position, replace the
695/40 filter with the 675/20 filter to detect PerCP.
If you want to install a custom filter or dichroic, the filter should comply with the
following specifications.
For the longpass filters, the surface that faces the center of the octagon/trigon
should be coated directly on its surface, not between two or more pieces of glass.
The coating should transmit >70% of the wavelength range you want the filter to
transmit, with a minimum transmission of >50%, and it should reflect >90% of
the wavelength range you want the filter to reflect.
The opposite surface (facing away from the center) should be coated with an
anti-reflective coating with a minimum reflection of ≤1% of the wavelength
range you want the filter to reflect.
Note that filters must be installed in front of each PMT to block unwanted laser
light. For the dichroic, carefully assembly the glass filter in the holder using the
retaining spring. Assemble the filter with the arrow pointing toward the center of
the octagon or trigon. The angle of the dichroic is critical to achieving optimal
results.
Note that any time you modify a detector array, you will need to create a new
cytometer configuration to identify which PMT (identified by laser color and
letter) will detect the emitted light. See Defining a Custom Configuration on
page 252 for instructions.
Table A-1 BD FACSAria filter specifications
Filter Characteristic Dichroic LP Filters BP Filters
Diameter 0.622 ±0.003 in. 1.00 ±0.010 in.
Thickness 0.125 ±0.005 in. 0.12–0.35 in.
Minimum clear aperture 0.562 in. 0.85 in.
Incident angle 11 ±1° 0°
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