Appendix B: Application Settings 263
2 Install the unstained control tube onto the cytometer.
3 In the Cytometer window, optimize the settings for your application.
• Optimize the FSC and SSC voltages to place the population of interest
on scale.
• Optimize the FSC threshold value to eliminate debris without
interfering with the population of interest.
• If needed, increase the fluorescence PMT voltages to place the negative
population within the gray boxes. Try to align the center of the
negative population with the crosshair shown in the gray box.
NOTE Do not decrease the fluorescence PMT voltages at this point. Doing
so may decrease your ability to resolve dim populations from the negative
population.
4 Unload the unstained control tube from the cytometer.
5 Load the multi-color sample onto the cytometer.
6 Verify that the positive populations are on scale.
If a positive population is off scale, lower the PMT voltage for that
parameter until the positive population can be seen entirely on scale.
7 Unload the multi-color sample from the cytometer.
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