34 BD FACSAria User’s Guide
Aerosol Management
During sample acquisition and sorting, the sort block door should be kept closed
to help contain potential aerosols (Figure 1-13).
Figure 1-13 Sort block with door closed
Additional aerosol removal is provided by the BD™ Aerosol Management
Option (AMO). The AMO is an optional device that evacuates the sort collection
chamber during sorting. The AMO is equipped with a 0.01-µm pore, ultra-low
penetrating air (ULPA) filter to trap aerosolized particles. The AMO pump
evacuates the sort collection chamber 16 times per minute when operating at
20% capacity in normal evacuation mode, and 69 times per minute at 100%
capacity in rapid evacuation mode.
Cell sorters that use droplet generation methods, like the BD FACSAria,
can produce aerosols around the sample stream. Inhalation or contact with
aerosols exposes you to biologically transmissible diseases. When acquiring
biohazardous samples, follow universal precautions at all times. Keep the
sort block door closed during sorting. If you need to access the sort block,
turn off the stream before opening the door.
sort block
sort collection
chamber
deflection plates
warning light
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