Chapter 1: BD FACSAria Cytometer Components 33
Deflection Plates
The high-voltage deflection plates are used to deflect side streams during sorting.
The plates are turned on and off using the Voltage control in the Side Stream
window (see Side Stream Formation on page 67). A red warning light is
illuminated whenever the plate voltage is on (Figure 1-13 on page 34).
Aspirator Drawer
The aspirator drawer keeps the sort collection tubes covered until sorting begins
(Figure 1-12). You can open and close the drawer using a control in the Sort
Layout or Side Stream window (see Using Sorting Controls on page 100). When
the Sweet Spot is on and a clog is detected during sorting, the drawer closes
automatically to protect your sort collection tubes.
Figure 1-12 Aspirator drawer closed (left) vs open (right)
A 12,000-volt potential exists between the deflection plates when they are
on. Contact with the charged plates results in serious electrical shock. Do
not touch the deflection plates when the voltage warning light is
illuminated, or when the software indicates that the plate voltage is on. The
plates remain energized even when the sort block door is opened.
To avoid pinching your hands or fingers in the drawer, keep your hands
away from the sort block during sorting.
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