72 BD FACSAria User’s Guide
Figure 2-17 Target particle within a Yield Mask of 16
If the Yield Mask were set to 8 for the same target particle, the target particle
would fall outside of the Yield Mask; therefore, no additional drops would be
sorted. See Figure 2-18.
Figure 2-18 Target particle outside a Yield Mask of 8
When the Yield Mask is set to zero, only one drop (the drop containing the target
particle) will be deflected; when the mask is set to 32, two drops will always be
deflected. Yield Masks between 0–32 will sort either one or two drops.
Yield Masks cannot be used in conjunction with Phase Masks. Therefore, when
the Yield Mask is greater than zero, the Phase Mask automatically reverts to
zero.
trailing drop: sorted drop being interrogated leading drop: not sorted
target particle
Yield Mask
Yield Mask
trailing drop: not sorted drop being interrogated leading drop: not sorted
Yield Mask
target particle
Yield Mask
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