Chapter 2: Theory of Operation 73
Purity Mask
The Purity Mask setting defines how close, in 1/32-drop increments, a
contaminating drop can be located before ignoring the drop being interrogated.
For example, when the Purity Mask is set to 16, the drop being interrogated will
not be sorted if a non-target particle falls within the first or last 8/32 of the
leading or trailing drop. In the following example, a non-target particle falls
within the first 8/32, so the interrogated drop will not be sorted (Figure 2-19).
Figure 2-19 Non-target particle within a Purity Mask of 16
If the Purity Mask were set to 8 for the same target particle, the non-target
particle would fall outside of the Purity Mask, so the interrogated drop would be
sorted. See Figure 2-20.
Figure 2-20 Non-target particle outside a Purity Mask of 8
NOTE With any Purity Mask greater than zero, the drop being interrogated must
be free of contaminating particles or the drop will not be sorted. If the purity
mask is set to zero, a droplet containing the event of interest will be sorted
regardless of contaminating particles.
trailing drop leading drop
Purity Mask
Purity Mask
non-target particle
(not sorted)
drop being interrogated
Purity Mask
Purity Mask
non-target particle
(sorted)
trailing drop leading drop
drop being interrogated
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