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22.5 Parameter Calculation
Leukocyte (white blood
cells) concentration
WBC = WBC
CAL
x WBC
MEASURED
Neutophil,
Lymphocyte,
Monocyte,
Eosinophil and
Basophil concentration
Calculated from differential percentage and WBC
NEU% x WBC
LYM% x WBC
MON% x WBC
EO% x WBC
BAS% x WBC
Neutophil,
Lymphocyte,
Monocyte and
Eosinophil five part
differential percentage
Relative value are derived from the optical 4 diff
scatter populations
Basophil five part
differential percentage
Relative value is calculated from the basophil
optical scatter populations
Erythrocyte (red blood
cells) concentration
RBC = RBC
CAL
x RBC
MEASURED
Measured photometrically at a wavelength of 568
nm; blank measurement is performed per cycle on
diluent and lyse mixture
HGB = HGB
CAL
x (HGB
MEASURED
– HGB
BLANK
)
Calculated from RBC and MCV
HCT = RBC x MCV x 100
Average volume of individual erythrocytes derived
from the RBC histogram
Mean Corpuscular
Hemoglobin
Average hemoglobin content of erythrocytes,
calculated from RBC and HGB values.
MCH = HGB / RBC
Mean Corpuscular
Hemoglobin Concentration
Calculated from HGB and HCT
MCHC = HGB / (HCT / 100)
Red Cell Distribution Width
Calculated from RDWcv and the distribution width
of the erythrocyte histogram at 20% of peak
RDWsd = RDWcv x (P
2
+ P
1
) / 0.56
Red Cell Distribution Width
CV
Derived from the distribution width of the
erythrocyte histogram at 20% of peak
RDWcv = RDW
CAL
x 0.56 x (P
2
- P
1
) / (P
2
+ P
1
)
Factor of 0.56 converts measurement at 60% of
peak to 20% of peak
Platelet (thrombocyte)
concentration
PLT = PLT
CAL
x PLT
MEASURED
Calculated from PLT and MPV
PCT = PLT x MPV x 100
Average volume of individual platelets derived
from the PLT histogram