80
Warning flags are raised when the analyzer detects a problem in the analysis process that may cause incorrect or suspect results or the
parameters in the valid blank measurement was too high.
Blood Detector malfunction (received signals are all zeros or not
received signals)
Check BD and its connection to the LSDACQ card. Check sampling
tube connection to BD.
The number of the BASO population is high or there are lyse-
resistant cells in the sample.
Repeat the measurement or perform a manual count on a stained
smear.
There is a (partial) clogging or leakage in the WBC part of
measurement system. A faulty/ worn-out pump can create
vacuum errors as well.
Perform cleaning of the ’Abacus 5’. Perform a self-test.
If vacuum problems persist then call for service.
There is a (partial) clogging or leakage in the RBC part of
measurement system. A faulty/ worn-out pump can create
vacuum errors as well.
Perform cleaning of the ’Abacus 5’. Perform a self-test.
If vacuum problems persist then call for service.
Blood Detector detected a sample volume that was too small.
Check sample volume in sample vial. Check sampling process. Re-
run sample.
Not enough cells detected during the 4DIFF WBC differentiating.
Perform a flow cell cleaning procedure! Repeat the
measurement. If the problem persists then ask for service.
Not enough cells detected during the BASO WBC differentiating.
If the total-WBC count is below 2 x 10
3
/µl then perform a manual
count on a stained smear.
Perform a flow cell cleaning procedure! Repeat the
measurement. If the problem persists then ask for service.
Differential percentage
error
Algorithm error while distinguishing the WBC-s in the 4DIFF
procedure.
Repeat the measurement. If the problem persists on a given
sample then perform a manual count on a stained smear.
Algorithm error while distinguishing the WBC-s in the BASO
procedure.
Repeat the measurement. If the problem persists on a given
sample then perform a manual count on a stained smear.
During WBC count there is too much (more than 10% of the total)
detected pulse on the first 10 channel of the 255 channel full
range. This may be caused by lyse resistant RBC, electronic noise,
contaminated WBC chamber or contaminated reagents.
Perform Clean process. Perform Hard Clean process.
Table 13. Warning Flags
Interpretive flags infer the presence of distributional abnormalities such as leukocytosis.