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Figure 6. Optical Signal Processing System
External structure and the size of the cell cause lower angles of scatter, and internal granularity or complexity causes
higher angles of diffraction. Both low and high angles of light scatter are captured by optical sensors, providing the
‘Abacus 5’ analyzer with two independent measurements for each cell crossing the path of the laser beam.
Figure 7. Cellular Light Scatter
The light scatter data are gathered and plotted as a two dimensional scatter diagram. Similar cells have similar
scatter characteristics and tend to group together. This allows the analytical software to differentiate and identify the
leukocyte populations and generate the 4DIFF and BASO scatter diagrams.
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